FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955656
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC2259.0932554E-725.782183
GCCCACC4650.024.946577145
TTACCCC2451.908229E-623.678754
CCGTGCA1851.8297648E-423.5150669
GTATAAG5550.022.2109991
CCTAAGA1650.002598199321.9734482
GAGCCCC5450.021.284697145
CTGTACG5151.8189894E-1221.11799
TATATAG4501.3096724E-1020.9491165
CACGAGC8400.020.714571145
CTCCGAC5850.019.829332145
GTCTTAG2205.8963156E-419.7761041
CCACGAC4853.601599E-1019.433256145
CCGTGCC1900.005885454419.0802069
GTTAAGG4954.7293724E-1019.0436531
CTACACT4202.8489012E-818.992334
TATATCG3451.7381317E-618.9172615
ATACGGG2307.9496583E-418.9172616
ACTCCGT3151.6801694E-518.4168056
GTATAAT7900.018.3575631
Produced by FastQC (version 0.11.2)