FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg25362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg25362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC90.509915014164306No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG80.4532577903682719No Hit
GTTTGGTACCCGTCTTCAAGGAGGAAAAGACAGTGCTTCTGCTCGTTACA20.11331444759206798No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCAAAGC20.11331444759206798No Hit
ATCAAGGATTCCAAGGAGAACGTGGGCGCTCGGGTCTTCCAGGACTTCCA20.11331444759206798No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCGTCGG20.11331444759206798No Hit
ACCAAGAACAAGATGAAAATGAAGAAAAGAAACCAGATGACGAGAAGAAA20.11331444759206798No Hit
TAGTGATTTTCTTCAATTGGGAGAACCAATTTACGTCACTAAAGACTTCG20.11331444759206798No Hit
ATTTCCAGTTGAGCGAAGTGAAAATTCAGAAAATATACCTAGATACAATT20.11331444759206798No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCGTAGGCTTAGG20.11331444759206798No Hit
GGTCACCAGAGGTCACCAGAGGTCACCAGAGGTCACCAGAGGTCACCAGA20.11331444759206798No Hit
GAGAGGGTGTCAGTCAAAGTACTTCGACAGGGCCTATTCAGGACCTGGAA20.11331444759206798No Hit
CATAGTTCTTTGCTTTTTGATTCGAAAGATCTTGTTGCGAAGGAATTGGA20.11331444759206798No Hit
CCTCGTTTCTCTCACAGTCAATTGTAATCCTTGAGTCTAGAGAATGTTTA20.11331444759206798No Hit
GCTTCTGCATCCCTGTGACCAAACACGCTCCATTGATAATCCGTTGGTTA20.11331444759206798No Hit
ATCATATTTAATTTCAAGTTTTCCGTTCGAAATGTTCCTTAACTTTCTAT20.11331444759206798No Hit
AGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAG20.11331444759206798No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCGTAGG20.11331444759206798No Hit
GAACTGTCAACGTGAATGCGAGGTCCAAGTGGGCCATTTTTGGTAAGCAG20.11331444759206798No Hit
CCTTAATTGTACGCTTGCGTATGTACCCCTTTATTGTACAATTTGGTATG20.11331444759206798No Hit
ATTCAAAAACATGTATCAGAAGATGACGAAGATACAGTTAATTCTGATGA20.11331444759206798No Hit
GTGTATAAGAGACAGACGGAAGAGAGAGAGAGAGAGAGAGAAGCTCTAGC20.11331444759206798No Hit
CCTCTTCGTGGCGTAGAAGATCAACACTCTGGGCGACCAGGTGGCTAAGA20.11331444759206798No Hit
TTGGAATAGTACAAATGAACGATCTTTCTGCAGAGAAAATCAAGGAAAAG20.11331444759206798No Hit
TTTTGTGTAGAACTTCCTACAGTTCCTTTCCATCTCTTGTAAGTGGGGTA20.11331444759206798No Hit
TCATAAACTTCTGGGCTATCCATGGCATCTTATGCTTGAATCGCGAGCTT20.11331444759206798No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)