FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg25361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg25361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408068
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT6000.025.3845121
GCAGCGT2401.5936475E-624.1757261
CCACGAC5350.023.037016145
CTATACA6400.022.6607194
TGCCGTG17700.022.52789145
TCTATAC6450.022.4850543
GCTACAC4659.094947E-1221.832263
CTACACG4358.367351E-1121.6709384
GCCCACC6400.021.523096145
GTATAAG7800.020.4563851
TAAGGCC3259.837058E-720.076603145
CTGCTTT14500.019.999683145
TATACAG7400.019.598465
CCCCTAG23050.019.5083941
TATACTA5605.456968E-1219.4234735
GTATTAG6000.019.340581
GTGTATA9400.019.2891451
CTAGTGA24450.019.277834
CCTAGTG21650.019.0915243
TAGTGAC24000.019.0350045
Produced by FastQC (version 0.11.2)