FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2535.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2535.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959690
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8650.029.336739145
CTATACT8050.021.6159934
CCACGAC9550.020.498415145
GTATAAG10300.020.4162541
TATACCG6050.020.3734475
CCTATAC5800.020.0010073
GCGAATG21800.019.28996145
GTGTATA12450.019.2202871
TAGGACA12900.019.109494
GGGCTAT3501.9996187E-618.6442726
CTCTAGG14350.018.1913911
GTATACG4806.661139E-918.1282251
CTGTACG12100.017.9747359
TGCCGTG26450.017.817533145
CTAGGAC11900.017.6689573
CCCACGC5751.8371793E-1017.65306145
CTGCTTT21750.017.334206145
TTATACT19800.017.2104634
TAGCACT5552.1791493E-916.9828364
GAGCGCG6003.3651304E-1016.9162249
Produced by FastQC (version 0.11.2)