FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194915
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC55200.25149037662050694No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG32190.14665715984445868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG12900.025.8599991
GCCCACC11750.022.212444145
GTGTATA13100.022.1436421
CGTTATG13150.020.4049871
CCTAGTG40550.019.6708263
CCCCTAG42050.019.4882491
CACAACG13850.019.3719233
TGCCGTG8250.019.333055145
TATACTG14900.018.9801275
CCTATAC6900.018.9166493
CACGAGC26100.018.888617145
CCACGAC13600.018.65782145
CCCTAGT42650.018.5326772
CCCTGTG18650.018.2746752
GTATATA14400.018.1301061
CCTGTGA18450.018.0793253
ACAACGT16300.017.7952084
CGTCTTG15200.017.64777145
CTGTGAC20100.017.3171274
GTATAGC4205.93378E-717.266771
Produced by FastQC (version 0.11.2)