FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567697
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7450.030.16823145
TGCCGTG5600.027.1879145
CCACGAC7450.025.302385145
CCCCTAG29150.024.8748971
CCCTAGT29800.024.0874612
CCTAGTG29200.024.0857963
GCCTTAC1650.002599363721.9728221
GTGTATA9400.020.827431
AGTGACC37000.020.5759416
CTAGTGA37500.020.4949444
GTATAAG7800.020.4516281
TAGTGAC36850.020.2661785
CAGCGTG3001.106343E-519.3330029
CCTTACA4202.8572686E-818.9895882
CCCACGC5005.4205884E-1018.850279145
CCTACAC3852.3852044E-718.8326473
ACAGGCC2400.001060197618.124698
GCAGCGT2851.6319813E-417.8095511
CGTCTTG11500.017.652433145
GTCCTAT2901.8641938E-417.5024911
Produced by FastQC (version 0.11.2)