FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017876
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG5700.033.072906145
GCCCACC4600.028.371912145
GTGTATA7250.027.0062121
GTATAAG6250.025.5258711
CCACGAC5300.021.888601145
ACTACGC2003.1034177E-421.7496628
GTATATA5700.021.627781
TAGAACC5151.8189894E-1221.1203254
TAATACT7300.020.859944
TAAGGTG2455.1069983E-520.7180335
TAGGACA6350.020.5549014
GAGCCCC5052.910383E-1120.100672145
TTACACT6550.019.927274
CTAGGAC5407.4578566E-1118.7996983
CTGCTTT10850.018.711224145
ACAGTGC3902.7320675E-718.5894538
GTTAAGG5107.039489E-1018.4846441
TATATAC7650.018.0097943
GTATTAC4857.556082E-917.9422721
ATAAGAG12550.017.9116083
Produced by FastQC (version 0.11.2)