FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550293
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG10150.029.2969041
GCTATAC2301.1010306E-625.2245863
GTGTATA9600.022.6649061
CTCCGAC10000.020.30055145
TATAAGA15500.019.6507662
TGCTATA3701.5664955E-719.6001832
TATACTG9650.019.5398835
CCCACGC10100.019.381714145
GTCCTAC2659.326514E-519.158261
GTATATA11350.018.5313071
CCTAGTG21750.018.3385623
GCCCACC8350.018.234028145
GAGCATG16350.018.180922145
CAGTGCG2801.4267782E-418.1249089
CTAGGAC5651.4188117E-1017.9697253
TAGGTCG2050.00911192917.6885415
TAGGACA5751.8371793E-1017.657784
CCCCTAG24500.017.4658551
CTACCCA4608.068855E-817.3424634
AGGACAG5451.7043931E-917.2990685
Produced by FastQC (version 0.11.2)