FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486597
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC33460.2250778119423085No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG22960.1544466994081113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAACG13200.026.3700183
ACAACGT13300.026.1726264
CAACGTT14150.025.6254395
CCCCTAG39750.024.2678151
GTATAAG7950.023.720421
CGTTATG12550.023.6949771
CCTAGTG37400.023.461563
TAGTGAC43200.022.9982385
AGTGACC44200.022.8060596
CTAGTGA44600.022.276324
CCCTAGT41200.022.0031642
CCTGTGA12900.021.923913
ACGTTTC28650.021.7627947
GCCCACC9500.020.606783145
TCACAAC17650.020.5445982
TAATACT8500.020.4762334
CAATGCG17750.020.4226679
CCCTGTG12700.019.9864962
ACAATGC20950.019.7256648
CTGTGAC15400.018.8363594
Produced by FastQC (version 0.11.2)