FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249314
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG5200.032.067043145
GCCCACC6850.029.63479145
CTCCGAC7050.021.595566145
CCACGAC5500.021.090734145
CCCCTAG17700.020.076081
CCCTAGT18000.019.7391072
CTTAGAC3001.1048176E-519.3354953
TATACCG3051.2741306E-519.018525
TACTGGG3051.2754508E-519.0162377
CTGTACG5056.202754E-1018.6632129
TAATACT7500.018.368724
CCTAGTG17500.018.230613
GTGTATA6001.6370905E-1118.129931
ATAGTAC4054.0569466E-717.9032343
GTAACAC4054.0569466E-717.9032343
TACCGTG2450.001217223217.7549557
CAGTGCA5151.5925252E-816.8930649
CTACACT5201.7915227E-816.732644
CTGGCCG2600.00181217416.730639
GTATTAG5703.092282E-916.5395851
Produced by FastQC (version 0.11.2)