FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510147
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC12200.026.741837145
CGCTATG16600.024.45786145
CTGTACG9350.024.0365079
CGCTACC7900.023.860788145
GTGTATA14850.023.4432471
GTATAAG12700.022.2722381
CCACGAC13950.021.827986145
TGCCGTG25650.021.198856145
GTATATA17000.020.4783651
CTCCGAC16250.020.076946145
CCTAGTG34550.019.5191083
TAATACT14300.019.2692134
CCCCTAG37900.019.1365551
GTCCTAG15300.018.9614491
CCCTAGT37850.018.7763522
GTATTAA10950.018.5458551
CTGCTTT28200.017.48229145
ATCTCCC15850.017.381725145
CGTCTTG34100.017.221428145
CGAGACC19050.017.126005145
Produced by FastQC (version 0.11.2)