FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307758
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG7550.028.82011
GTGTATA8300.024.4681471
CTGTACG12900.024.1648459
GCCCACC6550.023.245188145
GTATATA9200.022.8629021
CTTAGGT1700.003087109221.3308933
CCTTAAC13550.020.8743653
CCCCTAG18850.020.0084341
GTTAAGG10600.019.8432731
CTTAACT14400.019.6421994
TAAGACT6650.019.6308364
CCTAAGG2256.853284E-419.340013
CAGTGCA4502.9976945E-919.3318759
ACAATGC10650.019.0595958
GTATTAC4603.914465E-918.921021
CCTAGTG17100.018.6614133
GTATAGG3501.9919808E-618.650721
TTAACTG16000.018.584545
CTCCTGC9800.018.495625145
GTATTAG4806.6356733E-918.1326471
Produced by FastQC (version 0.11.2)