FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993544
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA6300.026.4759641
GCCCACC5100.025.589247145
CTAGGGT1500.001492608624.1712724
TGCCGTG6000.024.167624145
CAGTGCA3452.9649527E-923.1156949
TATACAG5050.022.9746765
TACCCCA4455.456968E-1222.8133375
CTAGACT4659.094947E-1221.8321174
CGTCTTG10100.021.535505145
CTATGGT1700.00308936321.3275934
CTGCTTT9000.020.945272145
GTATAAG5650.020.5348853
ATACCCC4351.9590516E-920.0038134
GCAGCGT2556.941858E-519.9077591
CCTACAC2556.947235E-519.9057543
GTATAGA4051.87556E-819.6970941
ATACCGC1850.00504129519.598336
GTCTATA4152.4816472E-819.2224661
TATACTC6800.019.1948345
GTACCGG1900.005877960519.0845071
Produced by FastQC (version 0.11.2)