FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAATCGGGTTCTTGTTCGAAAGAGAAGATAGAGATGGAAAAAGTCAG11.8518518518518516No Hit
ACAGTATCCTTTGGGGATACTGTAGAATGATGGAAGTCCAGCTGGAGGCA11.8518518518518516No Hit
GTCTGAACAGAGTTGTGACGAGAAACAGAAATTACCTATAGTGAGCCAAT11.8518518518518516No Hit
CAATAACATACATTATAAACAAAAAGGGATTATAGATTATAAAATAAAAC11.8518518518518516No Hit
TATCCATTGCGAATACTACAATGTACTCAAGAATCGACGGATCGTGAAAC11.8518518518518516No Hit
GCATCATAATTTGAAATTTAGTGAAAAATTGAATATATTCAGAAAAACGC11.8518518518518516No Hit
GTTATATTCAGGCTCATCCAAATGATAAATACTTGATTCGTTTCAACAAT11.8518518518518516No Hit
CTAGGGGTCACAGGGGTCACTAGGGGTCACCAGAGGTCACTAGGGGTCAC11.8518518518518516No Hit
TAAGTGTGTAAGTGTGTAAGTGTGTAAGTGTGTAAGTGTGTGGGGGTGTA11.8518518518518516No Hit
TCATAAGAGAAAAATAAGAGATAAGAGAAAAATAAACAAATGCACCATGC11.8518518518518516No Hit
ATATTGAATTGATTGAAAGTCACTGGAATCCATTCGACGATTCGGATTCA11.8518518518518516No Hit
GGCCTGAAGTCTTTTCAATATAAAGAAAAATTCTAGTGTTAAACCGACCT11.8518518518518516No Hit
CCTCTTGTTCATCGTAATTGCTCATTACATATTACGAGTCAGTGCTCCTT11.8518518518518516No Hit
GGGTACGGTATCCTTGAAGCGGGGTTAAACTAGAAAAGAAGAGGAGGGAA11.8518518518518516No Hit
GTGTATAAGAGACAGGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAA11.8518518518518516No Hit
CAAAAATTCTGAAAATCAGAACAAAATCAGTAAAATATAATTTTTAAAGA11.8518518518518516No Hit
AGATAGGGGAGCGTGCAGCTCGGGGTTTGTAGTTTTGTAGTCAAGTTATT11.8518518518518516No Hit
GTCCGTCTTCCAGGAGCCTCTCTCCGATTCCCATCAATCGCCTGTTGAAG11.8518518518518516No Hit
GGGTTACGAAACTAAAATAGGAAGTGTTGGGGGAGGTATAAATATAAAAT11.8518518518518516No Hit
ATTATTCTCTGTATCGTAGTCAACGACCATTCTCCCAAATTCCTGTCTCT11.8518518518518516No Hit
TTCTGAATGTGTATGGTTACTGAGGGCTAGAAAACTAACAAGGTCACAAA11.8518518518518516No Hit
GAGAGAGAGAGACATGTATAAAGTGCATGAGTCATGTATTTGTACATATT11.8518518518518516No Hit
CAAAAATTAGTGATGATTAAACTTTTCATACGAAGGATTAAGACTGCAAA11.8518518518518516No Hit
ATTCAAAATGGCCGATATCTATTCAATGGCGCTAGTTATCTGGGAAGTAG11.8518518518518516No Hit
ATCTATCTCTTCACATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT11.8518518518518516No Hit
GTACTTGAGACAACTTCTGAGGAAGACGTTGTTGGGATGTCTCTTCGTTC11.8518518518518516No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT11.8518518518518516No Hit
GTGGGCATGGGTACGGTGCTGATTGGCGATATGTGGATGCACGCTGGTGG11.8518518518518516No Hit
TTTGTACACAATGTTGGAAAGATTCTGGAGGTTTTGGAAGAATGAAAATC11.8518518518518516No Hit
CTACTCCTTTTTTCAGTCACTTCCTCAAACCGGGAGCTGTTCGAATCGAT11.8518518518518516No Hit
GTGTAGGTTGAACATTAGGCTTAACGGTAATTTTACTGATAATTTTTTGT11.8518518518518516No Hit
CTAGAAGAGGGAAGATAAAAAAGAGGAAAGTAATTCTAATGACCCAGTCT11.8518518518518516No Hit
CCTTCTCTACAGATAAAAACAGAGATAAAGAGATCTTTTATACTGATTTA11.8518518518518516No Hit
ATCACAAAATTTTAGATAAAAATATATAGATATATGAAGATACGATCACA11.8518518518518516No Hit
ATTCTGAAGCGATGTCAACACTGCTCCCTGAGCCGCACAGGCGTTATCCG11.8518518518518516No Hit
TCGGGGATATTGCTGGGTTCAAGGATCAGCTTTTGGGCATCTTGGACAAT11.8518518518518516No Hit
ATACGAAGTGAAGGACACACTGATTACTTGTCGATCATCTAGATATATTT11.8518518518518516No Hit
CCATTACAGGCTTCGCACATAGCTTCCATCTACACCAAACTACAGAAACC11.8518518518518516No Hit
CCATTTCCCTCTCTTTCCCCCTTTTTTTTGGTTCTGCTCGAAAGCATGTT11.8518518518518516No Hit
ACAAATGAATATGGAAAAATCAAAATTATCCAGGTCGGAAATGTATTCTG11.8518518518518516No Hit
GCTCTAAAGTAGGCCTACAGTAACCTTGACTAACCGCAAACAATATCCTC11.8518518518518516No Hit
GAACTGAACGACATCCGAAAAGAATATGGTGACTTGTTCAAATGTCTCTG11.8518518518518516No Hit
TGTCAGACGTGTATTCTCGGAAATCGAATATGGGTTCCGAATATGATTTA11.8518518518518516No Hit
CGGAAATATTGATTGAACTTCTTCGGAACGGCATGAACACCAGGAAGATC11.8518518518518516No Hit
GAATCTGAGTATTGTTATTCTACAGAATACTGTAACTGTCTCTTATACAC11.8518518518518516No Hit
ATCTATACGTCACCAAGTTCGATTTCTATTAAACACAATATTATCAGAAA11.8518518518518516No Hit
GTTACATATTGTGAAATTCAGACGGGGTGTTCACCATGCAAAATGGCTAG11.8518518518518516No Hit
TTTCTATTCTCAAGAAAAAGATGTTTATGCCAATTATTTTATGAGTCGGA11.8518518518518516No Hit
ATTCTTATCATTCCAATCTCTCCTCACCGTTTTTTTTTTGAGAATTTAAT11.8518518518518516No Hit
AATAATGGTCAAATAGAGGTCAATTAGAGGTCAAATAGAGGTCAAATAGA11.8518518518518516No Hit
AGAGGTTAGTGGGAAGAGAAAGAAGGGAGTAAAGGCGATTGAAGGGAGCC11.8518518518518516No Hit
GATTAAAAAAGAGGAATTGACCTGTAGAAAAGCTCAACAAGAGAGATTGA11.8518518518518516No Hit
GACAAACTGTGTGTGTGTCGATTCGATAGTCATGTTTATGGATGAGAATG11.8518518518518516No Hit
CTCTTCCTGCTCTTTCGAGAAGAATTCGAGAACGTCGATCCACTTTCACT11.8518518518518516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACA50.0145.08
GAGAGAC50.0145.07
GAAAGAG50.0145.0145
GAGACAT50.0145.09
AGAGAGA150.048.3333322
GAGAGAG150.048.3333321
Produced by FastQC (version 0.11.2)