FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2524.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2524.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144705
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10250.028.999617145
TGCCGTG14800.024.492922145
GTGTATA12650.023.507581
CTGTACG8550.022.0454659
CTGCTTT19500.021.935608145
CCACGAC9950.021.859007145
GTATAAG11300.021.8230291
CAGGCAC7000.019.678312145
CCCCTAG22200.019.6025641
GTATAAA18200.019.5271661
CGTCTTG21600.019.131693145
GTATAGA8400.018.9958171
GCCCTAA2701.0765392E-418.803941
CACTAGG18750.018.5675471
CTATAAG6301.8189894E-1218.4201871
CTCCGAC11850.018.354189145
CCTAGTG21500.018.2145843
TATACTC13400.017.8596555
CCCTAGT22700.017.5711732
TAACCTC8850.017.208385
Produced by FastQC (version 0.11.2)