FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222893
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC23330.19077711623175533No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG16060.1313279248470635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG5100.034.118587145
CGTTATG8450.028.3201831
GTATAAG6600.025.2710151
GCCCACC6050.025.165983145
CAATGCG12200.023.1759229
TAGTACG1902.1907184E-422.8991154
TATGACA12650.021.7828224
TATAATA12250.020.7182482
TTATGAC13300.020.7182463
GTATACG3551.01270416E-720.4272771
ACAATGC14050.019.6082828
GTTATGA13450.019.4089152
ACCTAAG2256.858153E-419.3378221
TATACTA5305.638867E-1119.1546045
GTATATA6850.019.0555171
CCCTGTG6500.018.9651642
GTTATAT7300.018.8742451
TTAATAC6600.018.6778133
CTATAAG3602.6118541E-618.1292081
ATATAAT16050.018.0727311
Produced by FastQC (version 0.11.2)