FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545695
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC18640.12059300185353515No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15920.10299573978048711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA2401.5770638E-930.2180082
TGCCGTG8900.028.51114145
GCCCACC7900.028.44927145
GTATAAG7350.027.6305751
TAGGACA5950.026.815314
GTGTATA8100.025.0721871
CTGTACG11900.023.7604249
ACAGGAC3854.274625E-1022.6039093
CTAGGAC5200.022.3141163
TATACCG3757.743438E-921.2734785
ACTGTGC3809.05311E-920.986778
CCCACGC4952.1827873E-1120.504982145
CCACGAC6500.020.076857145
CCTTAAC12300.018.8672183
CTTAACT14350.018.6993664
GTCCTAG5056.166374E-1018.6711561
TAGGTCG1950.006829570518.59575
TACATAG3902.7247188E-718.59575
CTCTAGG6650.018.5415691
CCTAAGG2751.2397088E-418.4604572
Produced by FastQC (version 0.11.2)