FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010588
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG7550.032.650208145
GTGTATA11600.023.1321771
CTATACT8450.022.3135054
GCCCACC10200.021.32431145
GTATAAG11350.021.0858361
CTCCGAC11550.020.715046145
CCCCTAG31900.019.7788661
TAATACT13050.018.8938124
CCTAGTG30550.018.7523733
CCCTAGT32200.018.6932242
CCACGAC9700.017.191353145
TAGTGAC36400.017.1335855
ACAGTGC9750.017.1019158
TATAGTA8950.017.0160312
CTAGTGA36250.017.004434
CATGATG33550.016.85606145
TCTATAC9150.016.6432673
GTTAAGG11500.016.3963931
GCCTTAC3103.0983175E-416.3760491
AGTGACC38600.016.3449336
Produced by FastQC (version 0.11.2)