FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923724
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC21330.11087869153787135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG11050.030.182598145
GTATAAG12950.026.3168741
GCCCACC8500.025.589592145
GTGTATA13100.024.9084911
CCACGAC10600.024.623947145
TGCCGTG16550.022.780664145
GCGTCAG5900.020.8931121
CTCCGAC11250.020.623318145
CGCTACC6350.019.410479145
CGTCTTG26250.019.334358145
TGTATAA16250.018.7404292
CTATACT6251.8189894E-1218.561954
TATACAG11000.018.4564845
ACAGTGC8000.018.1240798
CTAGGAC8500.017.9131813
GTATATA12600.017.8401031
GTATTAT15050.017.8267211
GTTAAGG13100.017.7127061
CTGTACG11200.017.476799
TATAAGA21200.017.442872
Produced by FastQC (version 0.11.2)