FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1992096
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC9950.034.24725145
GCCCACC7400.029.392769145
GTATAAG11350.028.7532861
CCTAGTG37250.025.6978473
CCCCTAG40900.024.4695871
CCCTAGT42050.023.6273252
GTGTATA11200.022.6631831
CCACGAC8050.022.5162145
CTAGTGA44750.022.3638214
TAGTGAC46700.022.0511595
AGTGACC47600.021.6342266
GAGACCC11900.021.324165145
CTGTGCG3406.4408596E-821.3230939
CCCTACA5251.8189894E-1220.7201022
GTCCTAA5750.020.1800861
GTCCTAT5356.548362E-1118.9777661
GTCCTAG6500.018.967341
CTGTACG12550.018.4856789
TGCTATG5901.2732926E-1118.437382
GCCCTAA2801.4236412E-418.1305471
Produced by FastQC (version 0.11.2)