FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100181
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG22820.1086573014421138No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC22270.10603847954057292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10250.029.707571145
GTGTATA13600.025.0684931
TGCCGTG7300.024.82898145
AGACCGC9500.024.42126145
CCACGAC10150.023.57163145
GCCCTAC1851.8250018E-423.5260351
GTATAAG12650.023.510541
CTAGCGT2908.225452E-620.0087453
CCCACGC9100.019.917751145
GAGACCC12700.019.409615145
CTATACT9150.019.024714
CCCCTAG25800.018.556391
GTCCTAG10650.018.3900721
CGTCTTG13100.017.710073145
CCTAGTG24700.017.6190383
ACCGTGC4155.2502764E-717.4696128
TATACCG5001.1006705E-817.407615
GTATATA13850.017.283571
GTATAGG6303.6379788E-1117.27111
CCTAGAG13550.017.129263
Produced by FastQC (version 0.11.2)