FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867428
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC45310.11715796648315106No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG39570.10231606121691211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG17150.024.9501671
GCCCACC25050.022.57493145
CTCCGAC19350.022.106022145
CCTAGTG73850.020.7197173
CTAGTGA88550.020.555664
ACAATGC45150.020.3924168
CCCCTAG78550.020.127781
TAGTGAC89700.020.049335
CCCTAGT77800.019.8546892
CCACGAC23400.019.829132145
CCCTGTG29850.019.6790352
CGAGACC30600.019.665104145
AGTGACC93750.019.4150036
TGCCGTG44150.019.212978145
GTATAAA29550.018.898131
GTGTATA20350.018.8884811
CAATGCG46300.018.7898359
CGTCTTG48300.018.763008145
GACAATG54900.017.9595267
CGTTATG34750.017.5311361
Produced by FastQC (version 0.11.2)