FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380426
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7150.022.307726145
GTGTATA8800.022.2491871
GTATAAG7850.022.1696533
TATACCG4251.4679244E-920.4735855
CCCTTAG2857.087743E-620.3553311
CTGCTTT15050.019.750862145
GTATTAC5707.2759576E-1219.0831241
CGTCAGA8150.018.685071
GTACTAA3501.9960135E-618.6469381
ATGCTAC4303.734567E-818.5505711
ATAACGT9850.018.4043774
CAGGACT3552.2866843E-618.383644
CCTATAC4755.846232E-918.3191343
CGTCTTG25150.018.16106145
TAGGGCA3201.9257675E-518.128314
GATGTGT12600.017.8399126
AGATGTG11400.017.8096265
ACCGTGC2851.6331094E-417.807698
CTAGGAC5301.1768861E-917.7862683
TAACGTT9800.017.7577025
Produced by FastQC (version 0.11.2)