FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240191
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC30270.24407530775501515No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17240.13901084591002513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCC2951.4551915E-1129.49072145
GTATATA9050.026.4432071
GCCCACC6750.025.777075145
GCAGCGT2951.4248144E-824.5825391
CGACCTC4900.023.6785536
CCTAGTG17400.023.3383433
CCCCTAG17550.021.900171
CCCTAGT18400.021.675852
TGCCGTG16400.021.218935145
TATACTA4301.6971171E-920.2369025
GTCCTAG7550.020.1707081
CTAGTGA20000.019.941784
TAGGACT2556.947725E-519.9062354
CTATAGG2608.0634934E-519.524211
CTTAAGG1900.00588136819.0830423
CTAGGAC8000.019.0353363
TAGTGAC20650.018.9629085
TTATACT12650.018.3438594
AGTGACC21400.018.298326
TGTCGGT5651.4188117E-1017.968462
Produced by FastQC (version 0.11.2)