FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880399
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG16900.044.18589145
GCCCACC16750.031.163828145
GTGTATA19700.024.6647111
CTGCTTT37300.024.29597145
CGTCTTG27900.024.166393145
GTATAAG18100.024.0403121
CCACGAC20050.022.78029145
CTGTACG11500.022.695699
CTAGGGT5850.022.3126144
TAGGGTG6150.021.2241955
CCCACGC14300.019.772505145
GTATTAG12250.019.5364321
GTCCTAG13500.019.3390961
ACCGTGC4152.4954716E-819.21698
TATAAGA33600.018.992042
TATACTA14700.018.2523275
CTCCGAC20650.017.554281145
ACACATA41500.017.294987145
CACGAGC44600.017.230747145
TTATACT29950.016.7065264
Produced by FastQC (version 0.11.2)