FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808894
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC9290.11484817540988064No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG8570.10594713275163371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA4650.048.355971
GTATAAG4750.036.6487351
CTGTACG6200.028.0637769
TGTATAA6100.024.9676172
TATACTG5900.024.5847425
TATAAGA7200.024.1749952
ACAGGCC1550.001808469323.386488
CTTAACT7800.022.315384
CCTTAAC7500.022.2409953
GTATAGT2303.12973E-522.0755521
TGCCGTG6300.021.86315145
CCCTAGT18600.021.8354782
CCCCTAG18650.021.7796331
CCTAGTG16950.021.39383
GTACCGT2755.193113E-621.0981776
CCTAAAC4301.6916601E-920.239533
TAGTGCA1800.004297789720.145834
CTAGTGA20200.020.1059364
TAAGAGA9200.019.7078764
GTCCTAA1850.005032412719.6037731
Produced by FastQC (version 0.11.2)