FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC18330.13102037928005267No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17930.1281612329782512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG9200.028.371445145
CAGGCAC5150.026.749348145
TAAGGTG2559.4758434E-825.5926785
GCCCACC6950.025.037632145
ATAGCGC2751.9664913E-723.731393
CACGAGC7550.023.047884145
GTGTTAG3851.0495569E-820.718621
CTAAGGA2455.1090432E-520.717884
TAGTGCG1750.0036595620.7141787
TACACAG6350.020.5547475
TATTAAG5403.6379788E-1220.1431052
TACGGGT2556.9507354E-519.9054164
GTGTATA7750.019.6492731
TATAGCG1850.005041759519.5986962
CGTCTTG15750.019.334614145
CTACACA9100.019.1241994
GTATAAC4603.925379E-918.9170021
TATACTG7750.018.7129235
CTAGGAC8150.018.6842233
GTACTAC4303.735113E-818.5503921
Produced by FastQC (version 0.11.2)