FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667828
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC27000.101205924819741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC13400.029.7565145
GTATAAG14800.027.436691
CCCACGC10100.020.81616145
GTATATA18650.020.6064131
GTGTATA15650.018.5332261
CTAGGAC8250.018.4567183
TAATACT18300.018.2261894
TAGGACA10000.018.1271344
CCACGAC13600.017.591343145
GTATTAG10950.017.2172811
TACACAG14000.017.0909755
CTATACT9400.016.9700814
GTATATG12000.016.919291
GTATAGA14700.016.7713091
TACCCCA8250.016.6986225
CTCCGAC15400.016.47674145
CCTAGTG34850.016.4366533
CACGAGC22300.016.25508145
TATAAGA26350.016.2353062
TATACCG7151.4551915E-1116.225385
Produced by FastQC (version 0.11.2)