FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCC4800.036.249607145
GCCCACC9350.032.56649145
GTATAAG9850.025.0349671
GTGTATA10950.021.8576851
CCTAAAG4058.039933E-1021.4875953
CTGCTTT22850.020.306126145
TGCCGTG37300.019.631153145
GTATTAG7850.019.4023321
CTCCGAC11850.018.966038145
TAGACAG9200.018.9193575
CCTAGTG24400.018.4273743
ACAGTGC6700.018.3962298
GCGAATG29250.018.34168145
GTATACG5551.10958354E-1018.2952941
CCACGAC10050.018.034632145
CGTCTTG43400.017.87423145
CCCCTAG25650.017.8138391
GCTACCC2851.630507E-417.8120843
GTACGTA2050.00911202317.688836
TAGGACA11950.017.6000164
Produced by FastQC (version 0.11.2)