FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230891
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG13500.033.295666145
GCCCACC15050.033.23857145
CCACGAC14400.027.186983145
CGTCTTG23800.026.196981145
ACCCTAC5050.025.8514961
GTGCTAG5300.024.6320861
CCCTACA5950.024.3772092
GTCCTAG21600.023.5043811
CCCCTAG45450.022.9791071
GTATAAG14000.022.2763981
CCTAGTG44500.022.0004333
ATCCTAC8850.021.3076041
CCCTAGT45950.021.1490172
TAGGACA20500.020.872244
CCTACAC9050.020.834463
ATGCTAC9500.020.6131651
CCTAGAG27200.020.5295453
CCCCTAC6850.020.1172031
CCCACGC15200.020.032515145
CTGCTTT30600.019.66466145
Produced by FastQC (version 0.11.2)