FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274066
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC30250.23742883021758684No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG20030.15721320559531454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC6600.021.969774145
TACACCG3004.546473E-721.75525
CCACGAC5750.021.434858145
GTGTATA7850.021.2487851
CCTAGTG16950.020.9645353
CCCCTAG18400.020.4956361
CGTCTTG10650.020.422606145
CCCTAGT18150.020.3775712
GTATAAG6850.020.1158751
TGCCGTG8050.019.813736145
GTATTAT8650.019.283581
CTGTACG9800.019.2332529
GTATATA9550.018.9850851
TTACGCG1950.006844523418.588359
GTATTAA6301.8189894E-1218.4185451
TAGTGAC20100.018.399925
GTATAGG3552.2834574E-618.3861181
CTAGTGA21450.017.9180364
GTATAAA9400.017.7449951
GCAGCGT2901.861352E-417.5055581
Produced by FastQC (version 0.11.2)