FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470308
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGACG554.049489E-665.933123
TAGGTTG1007.1486284E-1058.021145
GTACGGA400.006050356754.3716938
GGTCACG450.00963830248.330397
CGCTACC1002.732182E-643.497356145
ATACGAC855.3256936E-542.6626053
CTTTACA1201.964836E-742.307081
GTCTAAC700.001054177441.4436721
ACCTAGG700.001054177441.4436721
CTGCTTT4450.040.727856145
AAGGTTG1105.2451996E-639.559875
ATTACGC750.001479932838.6807633
GACAGCG750.0014830338.664317
GGCTGCA750.0014830338.664317
TAAGGAG1157.1168215E-637.8398785
GTCCAGG800.002031880736.2632181
AGGTTGG1001.3873502E-436.2632146
TATTAGG1406.5808854E-736.2632142
ACAGAGC1001.3908267E-436.2477958
CCGATGT1051.8528893E-434.521719
Produced by FastQC (version 0.11.2)