FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453448
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG8300.027.078058145
GTATAAG12550.026.5832061
GTGTATA14250.025.9566461
GCCCACC12050.025.871126145
CCCACGC8450.021.449503145
CCACGAC12100.019.772541145
CGTCTTG17050.019.559933145
GTCCTAG14000.019.1675661
GTATATA15800.018.8200191
ACAGTGC8150.018.6808038
CCTATAG4959.73705E-917.5813243
TATACTG15350.017.4811065
CTATACT10250.016.9809864
TGTATAA18650.016.7214532
GTATTAG9550.016.7075421
GTATAAA18950.016.457071
TATACCG5753.4760888E-916.3964965
CCTAGAG16500.016.2627263
TATAAGA21650.016.0793232
AGTACCG5904.8912625E-915.9796385
Produced by FastQC (version 0.11.2)