FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464988
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC606.795999E-660.4224931
GTGTATA3650.037.7433661
TAACGGT800.002035607436.2495964
GTATAAG3150.034.5234263
GTCCTAA900.003624538232.225331
ATCCCGC900.00362644832.2218636
CCGGCAA1153.1597225E-431.521399
GACCGTG1153.1597225E-431.521397
TGTATAA4000.030.8121572
GTACTAG2003.2452772E-729.0027981
TCCTATA1050.007704727.618742
TATAAGA4200.027.618744
ATAAGAG4900.026.6323575
CCTAGTG13650.024.963093
ATACCCC1751.2544083E-424.8568654
CCCCTAG14450.024.5871451
CCCTAGT14350.024.25062
CTAGTGA14550.023.917264
CATACCC1851.8287545E-423.513253
TAGTGAC14950.023.277335
Produced by FastQC (version 0.11.2)