FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313547
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG8050.027.92049145
AGACCGC12400.026.896353145
GAGACCC14900.025.789734145
GCCCACC11800.023.962923145
CCACGAC12850.023.13332145
CTGTACG13200.023.0677079
GTATTAG10800.020.8169561
GTATTAA11200.020.7210251
TAATACC10850.020.049574
CCCCTAG37600.019.8668331
GTATAAG10050.019.483951
CCTAGTG36550.019.4425053
CTACACT10100.018.6666134
CCCTAGT37700.018.6578982
GTGTATA14450.018.5700531
GTCCTAC3552.2849272E-618.3862611
CCTTAAC15150.018.1879833
TAGTGAC41000.017.8628645
ATAATAC20500.017.6860033
CACGAGC17350.017.551208145
Produced by FastQC (version 0.11.2)