FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309794
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG2001.0655689E-525.3757029
ACCTGCG2301.1032862E-625.2180887
CTGTACG3801.2732926E-1124.8033149
GTATAAG7400.024.5004671
GCAGCGT2451.9079343E-623.6804521
TATACTA5950.023.1589725
GCCCACC5950.021.93254145
GTGTATA7100.021.4499871
ATCTCCC6050.020.371687145
CTCCGAC7300.019.862803145
GTCTAAG2608.061461E-519.5249881
CTGTGCC3751.8087121E-719.3338689
TATAATA13150.019.3015332
CTATACA5356.548362E-1118.9782834
CCTATAC3451.7385737E-618.91793
TACCGTG2701.07926506E-418.7968167
TAACCCG2359.1815775E-418.5168065
GTATAAT10900.017.9640141
CTTAACC4455.5264536E-817.925953
GTCTTAC3252.1982354E-517.8514181
Produced by FastQC (version 0.11.2)