FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22.7027027027027026No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG11.3513513513513513RNA PCR Primer, Index 39 (96% over 30bp)
ATTTTATGCTGAACTTCGAGGGTGATAGGTTTTTCCTCGACGTTGGGACC11.3513513513513513No Hit
GGATAAAGGTATCGTCTGGAGAGCTTGAGCTGGTTGGTGTATCTGCTGTC11.3513513513513513No Hit
CTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTTTG11.3513513513513513No Hit
GAGAGAGTTGCAAAGAGAGATGAAAAGCAATAATAATGAATAAATGCAAA11.3513513513513513No Hit
TTGTTGCACACTTGGATTATAAAACGAGGTGGGGGAATTGGAATGAGCTA11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT11.3513513513513513No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGGCGTCTT11.3513513513513513No Hit
GAGTATAAATTATTTTTGCTGAAAAAATGTTTTCTCCAAAAAATCTTCCA11.3513513513513513No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCT11.3513513513513513RNA PCR Primer, Index 26 (96% over 27bp)
GCTGAAGGGACCCTCTCTTCTTCTTCTTCTTTCCTCCTTCTTCTTCTTTC11.3513513513513513No Hit
TCCTGATACTAAGCATCTACCAAAAATAACTGCCGATACCAATATTTTAT11.3513513513513513No Hit
TCCCAAGGAAGGAGAAAAAGAGCCGGGAGAAAAGGGGGGAAAAACAAAAA11.3513513513513513No Hit
AATTAAAGAAATTGAGGTCTAGTCTTGATGCATAGAAAAGAAGTGGTAAG11.3513513513513513No Hit
CTTTTTGCACTATGTTCTGCTCAAAAGAGTTGTCTTTTCTTCTTGTTCTC11.3513513513513513No Hit
CTTCATTCATTACGATAAAACAAATCTCACAGACAACATCGCTTTCTCAG11.3513513513513513No Hit
TTTTTATTATTCTGATCTCACGAGGTGACACTAGGGGTCGCGGCCGAGGA11.3513513513513513No Hit
GCTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGG11.3513513513513513No Hit
AACAATGATAATAAACAATTTGTTTCACCACATTAAAAAAATGGAATAAA11.3513513513513513No Hit
TATCTGAAGGCAGTTAAGTCAGTCGAGAATGTTTCTAGGAGCACCAGAGA11.3513513513513513No Hit
ATTACGCATCTAGTACAAACTTGTGAGGGAAAACGGAAAGATACATGTCG11.3513513513513513No Hit
CTCTTATACACATCTCCGAGCCCACCAGACGAGAGGAATATAGTATGCCG11.3513513513513513No Hit
TCATTATCCAAGATAAGCTGGGCTTCCGATTGGAGGAAAAGGGAAAAATG11.3513513513513513No Hit
AAGTTCCTCGGCGTATCTCGGTGCCTGTGTGCTCCAGATTTTTAAAAGAT11.3513513513513513No Hit
TGGTTCTGTACGCAATATGAATGGCCTTGCGTAAGTAATTACTTGACAGC11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGGATGCCGG11.3513513513513513No Hit
ATCCTTCAGTTGGACCAGATGGACAGATCCTACCAACCGATTCACAAGGA11.3513513513513513No Hit
GAGGTGAACTACCTCCTATAGAAGAGCCTATCAAGCAGCTATTTATACCA11.3513513513513513No Hit
CGGATGTACAATCGAAAAACCTATTCTAACAACGACACTTTACAACTGTT11.3513513513513513No Hit
ACTCAAAGAAGTTCGAAAAGTAAGGGAACTTGAGAAAAATTCCAGAACAA11.3513513513513513No Hit
GTGCAAGCTCCGCCCATTTACTACTGAATAACGATGATCGCTTCACAAAA11.3513513513513513No Hit
GTGATTAGAGGATTCTAAAGAGAGGAGGTAACATTCCCTTTCATCGTTTC11.3513513513513513No Hit
GAGTAGACTCTTGTTGAGTGCATAAAACTTCTAGTTTCCGGTTCACAAGA11.3513513513513513No Hit
GCCCAGAAAAGTCGATTTTCGCCTCTAAAAACTCCAAAAAAATCCCAAAA11.3513513513513513No Hit
AGTCTAAAGATTGACTTAGAAAATCCAGAAGATCCCCTAGGTGACGTTGA11.3513513513513513No Hit
ATAATATTTATTATTGAACTTTTTTTCAACCAATTTTAGTCATCATCATC11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGACGCAATCTCGTATGCCGT11.3513513513513513No Hit
TCGTTGTCGTTGTTCTCCATCCCGATTCCATCCATCCATCCATCCCGAAC11.3513513513513513No Hit
CAGATAGAGACATATAGAGACAACAAAAAAAACCAATAAAAAACTGAATA11.3513513513513513No Hit
GACAGAATAGCGCCGGACAAGCATCAACCAACTGGCCAATCGTTCCAACC11.3513513513513513No Hit
GAGTATTTGCCAGCTAACGGGGGAGGCATCTCCTGAGTGGCCCATGAGAG11.3513513513513513No Hit
ACGTTATTTTATCATCTATTCAAGCAAAGTTTTGGCCAACACAAGTGCCA11.3513513513513513No Hit
GATCTAATCCAGTCAATGGTCCTCCTGGTTTTGCGAATAAATGATTTCTG11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGCTCGGGTGTTGCGG11.3513513513513513No Hit
GCTCTATTGATATCCGGGATTTTCAGAGATGCTGTGTGGATTCATTGCGG11.3513513513513513No Hit
TGCCAGGGCAATTTCCTTCCCTTAAAAGAGTGGATCCTGGCCATTCCTAA11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCGGGGATGGCGG11.3513513513513513No Hit
CATTATCCTAATGGACTCATCATTTTTCATTTTTTCATTTTGCACATATA11.3513513513513513No Hit
CTTTTTATAATTAAAAATACAGCACACATAAATCCACAGGGCTCCCTTCT11.3513513513513513No Hit
GTATCTCGTTATTGCTGCGGTTAAAAAGATCGTAGTTGGATCTAGCTTAC11.3513513513513513No Hit
AGCCTGTGCATGAACCGACCATGAGTTCATACAAATCCAGCAGAACTGCT11.3513513513513513No Hit
GTATATTATTTTTTCCTCTGTTTCTCGTCACTTACCAAAATTTCAAATCC11.3513513513513513No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTCTTCCG11.3513513513513513No Hit
GAAAACAAAACTTGAATCAAAAAAATCACAAATAAACGTAATATAGGTAA11.3513513513513513No Hit
AGTAACCCCAAATGACCACAAGTGACCCCAAATGTTCTTTCTGTCTCTTA11.3513513513513513No Hit
CTGGTGTTCTGTTCTGAAAACCGAAACAAATATTCTTCAGTTATTCGAAA11.3513513513513513No Hit
CCGTAATATTGTCTCCTCGCTGCCAACAACCGCAGCCACATTGTAACATG11.3513513513513513No Hit
ACATTATTCAGCGTCTTTGCAATGATAAATTCAATGTCTCCGACTGCGAT11.3513513513513513No Hit
AACATAGACACTAAAAATAAAAACAAAGAAATTATAAAATTTTTAACATT11.3513513513513513No Hit
CACAAGGAGTCAGTCACTCTCAATGCTCAGAAGTTTATTGCTGTCTCTTA11.3513513513513513No Hit
GATAGTAGTATCGTCGATATGACATCAGAATAATCGTTGCTTCTTTTTCG11.3513513513513513No Hit
GGTTCTTTTGAATTTCAAATTTCTCTTTTTCTTTTTCTCTTCAGATGACG11.3513513513513513No Hit
AATATTTTTTTGAAATGAATGAAGTTTTTGAGAGCATTTGACCTGTCTCT11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT11.3513513513513513No Hit
ATCAAATAGTTTCGTATTAATTATTTCCGAACGCTATTTCTTTTTGAAGT11.3513513513513513No Hit
GAATTGTAAATGATTCAAGAATGCTGAAGTTTGCAAACGGAGTCACATTT11.3513513513513513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCGCGTATGCC11.3513513513513513No Hit
GGGTCACTAGGGGTCACCAGAGGTCACTAGGGGTCAACAGATGTCACAAG11.3513513513513513No Hit
AAAAATAAGTATATATGACTACAGAAGCCTTAAAAACTAGGCTCACATTC11.3513513513513513No Hit
TAGCAAAGAGAGCATTGGTGGCCCCCAGAATACAACTACGAGTCTTCGGA11.3513513513513513No Hit
CAGTTGACCCCAGAAAATGGTAATACTCGATGATGAGCAGATGACGGATC11.3513513513513513No Hit
GAACCCTTCTCCACTTCAGTCTTCAAGGATCGAACTTGAATATTTGCTAC11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGTC50.0145.0145
GATTGAC50.0145.09
AAGATTG50.0145.07
TCTAAAG50.0145.03
CTAAAGA50.0145.04
GTCTAAA50.0145.02
AGTCTAA50.0145.01
AGATTGA50.0145.08
AAAGATT50.0145.06
TAAAGAT50.0145.05