FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655034
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1950.044.625021
GCCCACC2800.038.83877145
CCTATAC1801.2887904E-732.229183
TGCCGTG2558.913048E-1131.274097145
GTCCTTA1100.00965902626.3693281
CCTACAT1409.978322E-425.8984473
CTATACT2259.0823414E-725.7833424
TATTAGG2001.0629559E-525.3804782
CCTGAGT1500.001493838324.166358
CCCATAG1600.002171627222.6611422
ACTGTGG2252.6500073E-522.555268
CTGTACG1952.613641E-422.3073989
TAAGGTG1650.002596654521.974445
GCCTCAC2003.1017337E-421.7497147
CCCCATA1700.003087800221.3281331
TATACTG3106.2242543E-721.0529335
GTATAAA4851.6370905E-1120.9323541
AATAGCC2455.1110677E-520.7140147
CCACGAC3901.2176315E-820.448448145
CTTAAAC2857.0785773E-620.355273
Produced by FastQC (version 0.11.2)