FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850399
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC44440.24016441859296295No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG29860.16137060169185133No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT24220.13089068898113326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG10650.027.9172921
GTGTATA10850.025.3976121
TGCCGTG16150.025.139732145
CACAACG15100.024.9720213
ACAACGT16450.024.2457624
CGTTATG18350.023.7112371
CCTGTGA20250.023.6344873
GCCCACC8900.023.623981145
TAGTGAC62550.023.4187165
CCCTGTG19850.023.3801212
AGTGACC64350.023.3271056
CAACGTT15900.022.8040475
CCCCTAG49600.022.6615221
CTAGTGA64800.022.6055664
CCTAGTG48400.022.4736273
CTGTGAC21850.021.904414
CCCTAGT51050.021.7331662
CAATGCG27100.020.5994139
ACGTTTC33200.020.308527
TATACTG12300.020.0453135
Produced by FastQC (version 0.11.2)