FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549885
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC6420.11675168444311083No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG6150.11184156687307346No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG800.002033826436.2572335
GCCCACC2404.3655746E-1133.232773145
CTGTACG3650.029.7923369
GTACTAA1753.7593672E-629.011061
ATTATAC5250.024.86213
GCATAGA1600.002169098722.6648941
GTACTGG1650.00259363421.9780771
TACACCA1650.002596375521.974085
AGAGCGT1650.002596375521.974086
CCGTGTA1650.002600492421.9680849
ACTCTAG2353.6943828E-521.6039851
ACAGAGT2704.44167E-621.4799068
GATCTAG1700.003084213221.3316631
AGGGAGT1700.003087470321.3277846
CCTAGTG12450.020.9680373
CCCTAGT12950.020.7203032
TTGGGCA1750.003652441820.7184184
TAGTGAC15500.020.5847515
TAACACT3208.4402564E-720.3946934
GTATAAG2505.9507394E-520.3077451
Produced by FastQC (version 0.11.2)