FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391905
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG15140.10877179117827725No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC14800.10632909573570035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG7100.029.613688145
GTATAAG7150.028.4048251
GCCCACC7400.027.433369145
CTGTACG3751.0913936E-1125.1323879
AGACGGC12850.024.825752145
GTATTAG4950.023.445251
TAGGACA4950.023.4418814
GTGTATA8800.023.078921
CCTAGTG19450.022.745923
GTCCTAG4850.022.4331191
GTAGGAC1952.6091366E-422.3156723
CTGTGCG2404.3657295E-521.1450379
CCCCTAG21000.021.0693261
TGCCGTA2556.9581554E-519.902641145
CCCTAGT21800.019.6285782
GTCCCGT1850.005038472419.6008996
TACACTT9650.019.5399635
TAGTGAC25250.019.2438135
TAACACT7450.018.495884
CTAGTGA25100.018.2030664
Produced by FastQC (version 0.11.2)