FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256652
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG27650.030.153315145
GCCCACC9450.029.153059145
CTGCTTT23000.028.68441145
GTATAAG9000.027.3970321
CTATACT7100.022.4699754
CCCACGC6200.022.217455145
CCTAGTG24550.022.1528073
CCCCTAG25450.021.9416891
GTGTATA11450.021.5347841
CCCTAGT26050.021.1555772
GTCCTAC2755.202046E-621.097181
CTCCGAC12100.020.371637145
TAGGGGT29000.020.2546734
TGTAAGA12700.019.984042
CTAGTGA26500.019.702664
ACTGCGT4452.6102498E-919.5498758
ACTGTGC7050.019.538328
TAGGCCC3001.1043301E-519.3377954
TAGTGAC27000.019.3377955
ACTAGGG27400.019.3192942
Produced by FastQC (version 0.11.2)