FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645959
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG9300.032.743877145
GCCCACC7550.028.809654145
GTATAAG7550.025.9334161
GCCTTAC2001.0640182E-525.3811321
GTCTTAG3406.4213964E-821.328681
CCACGAC8250.021.09216145
TATAAGA14200.020.4249882
CCTAGTG13750.020.0387693
ACTGCGT2908.25491E-619.9999718
GTATATA11300.019.8941881
CGTCTTG16450.019.834002145
CTGCTTT16250.019.631933145
CTCCGAC10500.019.33448145
CCCCTAG13300.019.0835571
GTATTAG6151.8189894E-1218.8663441
CTATACA7800.018.5919764
CAGACCG3151.6824233E-518.4154685
CTAAGGT3552.288596E-618.3824883
CTACACA9350.017.8363674
TACTGCG2450.001217390617.7550757
Produced by FastQC (version 0.11.2)