FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671952
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8000.033.531216145
TGCCGTG5650.028.23006145
CTATACT6950.025.0449334
CCTATAC5150.022.5323353
GTATATA11000.022.4171121
CCACGAC7650.021.797363145
TCTATAC9200.021.284793
TGGACAG7200.021.1527715
CATACCG4151.0895747E-920.9707345
TAGGTTG4851.6370905E-1120.93475
GTCTTAC4201.2641976E-920.72171
ATCTCCC9150.020.60107145
GTATAGT5650.020.5383241
GTACTAA5103.274181E-1119.9090841
TATTAGG7300.019.8701252
GTCCTAA4752.728484E-1019.8492091
GTATATG6950.019.827241
GTATAAG7700.019.7798061
GTGTATA9900.019.7798041
CACGAGC15100.019.68541145
Produced by FastQC (version 0.11.2)