FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511045
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG8000.028.0983221
GCCCACC6400.026.054615145
GTGTATA8800.023.8959331
CCCACGC3952.3646862E-1123.860695145
GTATTAG5500.022.4127391
CCACGAC6550.022.137342145
TGCCGTG24850.020.71423145
TAAGGCC2857.1020713E-620.350822145
GCGAATG18600.019.878979145
CGTCTTG26900.019.405151145
GTTAAGG12150.019.097761
CCTATAC5005.4205884E-1018.8530673
GTCCTAA3201.9262929E-518.127951
TACCCTT6503.6379788E-1217.849655
CTGTACG9400.017.7393139
CTGCTTT16000.017.671827145
CCCCTAG21350.017.6609541
GTAGCAC3804.378675E-617.1738473
GTATATG8150.016.904591
TGTATAA11200.016.8330972
Produced by FastQC (version 0.11.2)