FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201884
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG4450.032.5834145
CTAGATG12200.024.95835145
GCCCACC5000.024.649342145
ACTGGCC1902.1939228E-422.8941258
GTACAGG2552.6996713E-622.7530121
CCTCGCG1952.615642E-422.3070969
CTTAAGG2003.09974E-421.7539442
CTGCTTT12700.021.692333145
GAGCCCC6100.021.39287145
GTATTAC4851.6370905E-1120.9351161
CTTAATA5251.8189894E-1220.7180422
CCTACAC2806.0823986E-620.7180423
TCCTACA5301.8189894E-1220.5225892
CCACGAC5700.020.350334145
GTATAAG5750.020.1809311
ACCTGCG1800.004308234420.1383517
CCTGCGC1800.004308234420.1383518
GTATTAG4351.9554136E-920.0069581
GTCCTAG3351.3104873E-619.4843881
ATCTCCC7550.019.204786145
Produced by FastQC (version 0.11.2)