FastQCFastQC Report
Fri 3 Mar 2023
H7NTWBGXN_n01_daplib_ShaAmpPoolRb1-input_z.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NTWBGXN_n01_daplib_ShaAmpPoolRb1-input_z.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15713961
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1766001.1238414044682943TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC1578911.0047816715339946TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)