FastQCFastQC Report
Fri 3 Mar 2023
H7NTWBGXN_n01_daplib_KyoAmpPoolRb1-input_z.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NTWBGXN_n01_daplib_KyoAmpPoolRb1-input_z.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17096948
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3092511.8088082153610106TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC729140.4264737776590301TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT253090.1480322686832761TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTAT192630.1126692319588268TruSeq Adapter, Index 27 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)