FastQCFastQC Report
Fri 3 Mar 2023
H7NTWBGXN_n01_daplib_CviAmpRb-input_z.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NTWBGXN_n01_daplib_CviAmpRb-input_z.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5625259
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT691331.2289745236619327TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC157090.2792582528200035TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)