FastQCFastQC Report
Fri 3 Mar 2023
H7NTWBGXN_n01_DEL2_KyoAmpPoolRb1-B_z.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NTWBGXN_n01_DEL2_KyoAmpPoolRb1-B_z.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33265825
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG4332551.302402691050049TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT1578530.474520021673895TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG666510.20035877661233412TruSeq Adapter, Index 6 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)